Nutritional (video) Microscopy

Nutritional (video) microscopy of Live & Dried Layered Blood Analysis is a unique technique used to formulate an appropriate course of natural health-building and lifestyle principles to optimize health, prevent disease, and to monitor individual effectiveness.

Live Blood Analysis and Dried Layered Blood Analysis are the two applications that are discussed in the following information. The two applications are modeled through three viewing techniques: Phase Contrast & Dark Field (Live Blood), and Bright Field (Dried Layered Blood), also known as the Oxidative Stress Test or Mycotoxic / Oxidative Stress Test (M.O.S.T).

The test is different from conventional blood tests ordered by the physicians because it is a live sample, where the qualified Analyst is looking for microbial activity, condition of cells, and anomalies that are not typically ordered in blood testing using the traditional method. The dried sample suggests the areas of the body that may be congested, or holding toxins, impairing proper functionality.

Phase Contrast Microscopy

An image of fibrin spiculae which should not appear in live blood at all. It indicates that the balance between haemostasis and fibrinolysis is too much in favor of clotting.

Around 1930 Professor Fritz Zernike, a Dutch professor in Physics, discovered phase and amplitude differences between 'zeroth order' and 'diffracted' light that can be altered to produce favorable conditions for interference and contrast enhancement through microscopy. He succeeded in devising a method, now known as phase contrast microscopy, for making unstained, phase objects yield contrast images as if they were amplitude objects.

Phase contrast microscopy was very successful and ultimately gained widespread application, resulting in Zernike's award of the prestigious Nobel Prize in physics in 1953. Phase contrast, by "converting" phase specimens such as living material into amplitude specimens, allowed the observing scientists to see details in unstained and/or living objects with a clarity and resolution never before achieved.

In Nutritional (video) Microscopy this unique technique of viewing living blood is not a diagnostic procedure for any specific disease. It is more a screening test to reflect how one’s dietary and lifestyle habits may be influencing health, and where appropriate adjustments are necessary within these areas in order to optimize health and prevent the onset of disease. Health problems and degenerative conditions can be prevented with early nutritional intervention, and Phase Contrast Microscopy can detect many nutritional imbalances and deficiencies before chemical blood tests can show abnormalities.

This unique Phase contrast technique of viewing living blood is different from regular blood analysis because it uses whole, unaltered blood as opposed to just parts of the blood. It is unstained and uses higher magnification. The blood which is viewed directly by the Analyst and the client/volunteer is alive, and not dead as in conventional chemical microscopic blood evaluation.

This Phase Contrast image of blood containing colonies of yeast markers have proven antibody positive for Candida Albicans (Drs. Majid Ali and Robert Bradford, Capital University, Washington DC, USA 1994).

Techniques of Live Blood Analysis (Phase Contrast & Dark Field Microscopy) can also view living microbial activity and their potential degenerative effects within the bloodstream. The presence of bacteria, fungi or parasitic forms observed in a live blood screening test is not diagnostic of an infection with any of these organisms. The blood and immune system is exposed to these organisms on a daily basis from the intake of food, municipal tap water, and the polluted environment of modern life. These organisms, when they enter the blood stream are generally inactivated by the immune system’s army of white blood cells and antibodies.

The mere presence of these ‘bugs’ in the blood is not diagnostic of an infection. For a blood infection to be present, a great deal more than just microbial activity has to be observed. Microbial activity within the bloodstream does however give indications of an acidic compromised biological terrain suited for the growth and development of such microbial activity and their excreted exotoxins, and therefore unsuited for efficient cellular function which requires a balanced alkaline biochemistry.

Skeptics of Phase Contrast and Dark Field Microscopy believe that the blood of most breathing, walking and functioning humans is completely sterile and that viruses, bacteria, fungi and parasites could not possibly exist in the bloodstream. They argue that if parasites, candida yeast, fungi or bacteria were really present in the bloodstream that the patient should be lying in a hospital bed, perhaps dying of septic shock. This dogma has been disproved by a great deal of research done by many scientists around the world, especially in Germany, Eastern Europe, New Zealand and countries where natural or drugless forms of medicine are more accepted.

The list of research papers describing the presence of viral, bacterial, fungal, and parasitic toxins in the blood of non-septicemic individuals is voluminous. A growing number of pathologists (e.g. Dr. A. Ali) and clinicians are recognizing the importance of using this kind of information in daily practice of preventive health care.

Dark Field Microscopy

Everybody is familiar with the appearance and visibility of stars on a cloudless night. They can be seen because of the contrast between their faint light and the black sky forming a background. Of course stars shine both night and day; it is just that they are invisible during the day because the overwhelming brightness of the sun obscures the faint light from the stars.

AT left is a Dark Field microscopic image of agglutinated erythrocytes (red blood cells).This is due to several imbalances within the diet as wells as emotional stressors.

It lowers oxygen to the tissues reducing cellular efficiency, inhibits efficient nutrient utilization, hinders the efficient removal of metabolic waste, and makes the circulatory and lymphatic systems sluggish.

This same principle is applied in Dark Field Microscopy which is a unique technique for making unstained objects visible. Dark Field illumination blocks out of the central light which normally passes through and around the specimen, disallowing any light other than oblique rays from every azimuth to illuminate the specimen on the microscope slide. In this way a situation is created as described above with the stars. They become visible against a dark background when lit obliquely. Objects are now observable using Dark Field Microscopy that cannot be observed in the Phase Contrast Microscopy application.

This Darkfield Microscopic image (at left) show corrugated red blood cell walls. This is partly due to lipid peroxidation of their bilayer phospholipid membranes. The white dots on these poikilocytic RBCs are mycoplasma.

Mycoplasma proliferation is implicated in many pathologies; mycoplasma feeds on sugars and produces several forms of sialic acid as metabolic end-product.

These are pathogens that infect plants, animals and humans. They are members of the mollicute family, cell-wall deficient and characterized as a virus-like infectious agent (in-between a virus and bacteria in complexity). There are hundreds of different mycoplasma subtypes and numerous isolates within any given subtype. For example, mycoplasma arthritidis can cause arthritic conditions, whilst mycoplasma pneumoniae is involved in respiratory conditions.

Mycoplasmas have a special interaction with the lymphoreticular system in that they are immuno-modulating pathogens that can compromise T-lymphocytes. By the time mycoplasma becomes visible in a live blood sample there is an urgent need to restore the client’s immune-competence.

Mycoplasma is a single cell bacterium that tends to make sore, achy muscles, pneumonia, arthritis, and lupus type symptoms. 60 days of super strong antioxidants inclusive of Beta-Carotene, Vitamin C, Vitamin E, Selenium, Zinc, Grape Seed Extract, Sea Kelp, combined with amino acids I-Cysteine, I-Glutathione will be of great benefit.

Dried Layered Blood

The coagulation in this slide above shows strong fibrin interconnections and no disseminated intravascular coagulation typical of a normal healthy dried layered profile . In Dried Layered Blood Analysis (coagulation morphology / Bright Field) one examines the result of the client’s/volunteer’s coagulation cascade. This is seen through cellular oxidization and degeneration, which is characterized through the fall out of fibrin toxic masses, gathered from one droplet of blood and collected in layers on the slide. This application of viewing dried suspended blood samples offers the qualified analyst and client valuable clues to potential degenerative patterns. Through the oxidation of the blood cells, and toxins present in the blood we are able to see characteristic patterning of an alternative pathway other than the extrinsic or intrinsic pathways. This allows us to identify what parts of the body are holding toxins and therefore functional capacity may be impaired. This technique may often present profiles that warrant the analyst making recommendations that the client visit their primary care physician for additional tests.

Coagulation in this slide shows a profound absence of fibrin network which reflects an amino acid deficiency caused either by an ingestion, digestion or assimilation problem	The above slide is a profile typical of individuals with chronic digestive irregularities and bowel toxicity.	This profile above is indicative of mental / emotional stress which indicates potential effects on the functional efficiency of the adrenal glands.

Since the systems within the human body are all interconnected, functioning as a whole, then by viewing the living elements of human blood using 'Phase Contrast,' 'Dark Field' modalities, one can observe biological imbalances that can have implications on the functional efficiency of various biological systems and the organs that comprise the system. By using the 'Bright Field' modality (dry layered blood) of analysis, one can gain insight into degenerative patterns and indications in various areas of the body.

Nutritional (video) Microscopy Can Show:

• The conditions of the red blood cells, white blood cells and platelets, and their implications on health.
• Nutritional deficiencies and indications of inefficient absorption and assimilation.
• Features associated with blood sugar imbalances.
• Features associated with crystalline forms such as cholesterol, triglycerides, uric acid, protoplast.
• Free radical activity and cellular oxidation.
• The presence of microbial activity such as bacteria, yeast, parasites, fungi.
• Levels of toxicity and internal pollution.
• Organ stresses or weaknesses such as: pancreas, liver, kidneys, prostate, heart, breast, reproductive.
• Features and early warning signs associated with degenerative conditions.

Copyright © 2001 InnerLife Wellness Center

REFERENCES
Ali, Majid. RDA: Rats, Drugs and Assumptions. Denville, New Jersey:Life Span Press, 1996 p.424-462.

Martin, Jeanne Marie and Rona, Zoltan P. The Complete Candida Yeast Guidebook. Rocklin, California:Prima Books, 1996.

Rogers, Sherry A. Finally Healing the Immune System. Macrobiotics Today. September/October 1995; pp. 16-20.

Rona, Zoltan P. and Martin, Jeanne Marie. Return to the Joy of Health, Vancouver: Alive Books, 1995.

Rona, Zoltan P. Childhood Illness and The Allergy Connection. Rocklin, California:Prima Books, 1996.

Simpson, L.O. Red cell shape changes following trigger finger fatigue in subjects with chronic tiredness and healthy controls. NZ Med. J. 1993; 106:104-7.

Simpson, L.O. Nondiscocytic erythrocytes in myalgic encephalomyelitis. NZ Med. J. 1989; 102: 126-7.